Review

rabbit polyclonal anti-vglut2 antibody (Synaptic Systems)

Synaptic Systems is a verified supplier

Synaptic Systems manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Synaptic Systems rabbit polyclonal anti-vglut2 antibody

Bmal1 deficiency affects the vesicular glutamate transporters (vGLUT) in the retina. (A) Representative photomicrographs of vGLUT1-immunoreaction (green) in retinal sections of Bmal1+/+ and Bmal1–/– mice. Quantitative analysis of immunoreaction (Ir) of vGLUT1 in the (B) OPL and (C) IPL. (D,E) Representative photomicrographs of <t>vGLUT2-Ir</t> (red) in retinal sections from Bmal1+/+ and Bmal1–/– mice. Quantification of vGLUT2-Ir in the (F) OPL, (G) IPL and (H) RGCs. Scale bars = 50 μm. Unpaired- t -test, * P < 0.05.

Rabbit Polyclonal Anti Vglut2 Antibody, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vglut2%2C+rabbit+polyclonal+antibody/pmc11903712-70-19-24?v=Synaptic+Systems
Average 90 stars, based on 1 article reviews

rabbit polyclonal anti-vglut2 antibody - by Bioz Stars, 2026-08

90/100 stars

Images

1) Product Images from "Loss of Bmal1 impairs the glutamatergic light input to the SCN in mice"

Article Title: Loss of Bmal1 impairs the glutamatergic light input to the SCN in mice

Journal: Frontiers in Cellular Neuroscience

doi: 10.3389/fncel.2025.1538985

Figure Legend Snippet: Bmal1 deficiency affects the vesicular glutamate transporters (vGLUT) in the retina. (A) Representative photomicrographs of vGLUT1-immunoreaction (green) in retinal sections of Bmal1+/+ and Bmal1–/– mice. Quantitative analysis of immunoreaction (Ir) of vGLUT1 in the (B) OPL and (C) IPL. (D,E) Representative photomicrographs of vGLUT2-Ir (red) in retinal sections from Bmal1+/+ and Bmal1–/– mice. Quantification of vGLUT2-Ir in the (F) OPL, (G) IPL and (H) RGCs. Scale bars = 50 μm. Unpaired- t -test, * P < 0.05.

Techniques Used:

Bmal1 deficiency affects the vesicular glutamate transporters in the core region of the suprachiasmatic nucleus (SCN). (A) representative photomicrographs of vGLUT1-immunoreaction (green) in the SCN core region of Bmal1+/+ and Bmal1–/– mice. (B) Quantification of the fluorescent vGLUT1-immunoreaction (Ir) in arbitrary units (a.u.). (C) Number of vGLUT1-immunoreactive particles per SCN. (D) Representative photomicrographs of vGLUT2-Ir (red) in SCN core region of Bmal1+/+ and Bmal1–/– mice. (E) Quantification of fluorescent vGLUT2-Ir. (F) Quantification of the number of vGLUT2-immunoreactive particles. Immunoreactive particles were analyzed in a 46.18 μm 2 area. N, DAPI-stained cell nuclei (blue). Scale bars = 10 μm. Unpaired- t -test, * P < 0.05, ** P < 0.01.

Figure Legend Snippet: Bmal1 deficiency affects the vesicular glutamate transporters in the core region of the suprachiasmatic nucleus (SCN). (A) representative photomicrographs of vGLUT1-immunoreaction (green) in the SCN core region of Bmal1+/+ and Bmal1–/– mice. (B) Quantification of the fluorescent vGLUT1-immunoreaction (Ir) in arbitrary units (a.u.). (C) Number of vGLUT1-immunoreactive particles per SCN. (D) Representative photomicrographs of vGLUT2-Ir (red) in SCN core region of Bmal1+/+ and Bmal1–/– mice. (E) Quantification of fluorescent vGLUT2-Ir. (F) Quantification of the number of vGLUT2-immunoreactive particles. Immunoreactive particles were analyzed in a 46.18 μm 2 area. N, DAPI-stained cell nuclei (blue). Scale bars = 10 μm. Unpaired- t -test, * P < 0.05, ** P < 0.01.

Techniques Used: Staining

Image Search Results

Journal: Frontiers in Cellular Neuroscience

Article Title: Loss of Bmal1 impairs the glutamatergic light input to the SCN in mice

doi: 10.3389/fncel.2025.1538985

Figure Lengend Snippet: Bmal1 deficiency affects the vesicular glutamate transporters (vGLUT) in the retina. (A) Representative photomicrographs of vGLUT1-immunoreaction (green) in retinal sections of Bmal1+/+ and Bmal1–/– mice. Quantitative analysis of immunoreaction (Ir) of vGLUT1 in the (B) OPL and (C) IPL. (D,E) Representative photomicrographs of vGLUT2-Ir (red) in retinal sections from Bmal1+/+ and Bmal1–/– mice. Quantification of vGLUT2-Ir in the (F) OPL, (G) IPL and (H) RGCs. Scale bars = 50 μm. Unpaired- t -test, * P < 0.05.

Article Snippet: Parallel retinal and brain sections were incubated with guinea pig anti-vGLUT1 (1:4000, Synaptic Systems, cat. #135304, Göttingen, Germany) or rabbit polyclonal anti-vGLUT2 antibody (1:3000, Synaptic Systems, cat. #135402 Göttingen, Germany).

Techniques:

Journal: Frontiers in Cellular Neuroscience

Article Title: Loss of Bmal1 impairs the glutamatergic light input to the SCN in mice

doi: 10.3389/fncel.2025.1538985

Figure Lengend Snippet: Bmal1 deficiency affects the vesicular glutamate transporters in the core region of the suprachiasmatic nucleus (SCN). (A) representative photomicrographs of vGLUT1-immunoreaction (green) in the SCN core region of Bmal1+/+ and Bmal1–/– mice. (B) Quantification of the fluorescent vGLUT1-immunoreaction (Ir) in arbitrary units (a.u.). (C) Number of vGLUT1-immunoreactive particles per SCN. (D) Representative photomicrographs of vGLUT2-Ir (red) in SCN core region of Bmal1+/+ and Bmal1–/– mice. (E) Quantification of fluorescent vGLUT2-Ir. (F) Quantification of the number of vGLUT2-immunoreactive particles. Immunoreactive particles were analyzed in a 46.18 μm 2 area. N, DAPI-stained cell nuclei (blue). Scale bars = 10 μm. Unpaired- t -test, * P < 0.05, ** P < 0.01.

Techniques: Staining

a Schema of rAAV 1/2 -pEF1α-DIO-GFP injection in the PAG of OTR-Cre rats. b Graph showing the relation between the number of OT fibers in the vlPAG and the bregma level. Blue area represents SEM. n fibers = 290, n rats = 4. c Graph showing the relation between the percentage of OTR neurons in the vlPAG and the bregma level. Blue area represents SEM. n cells = 528, n rats = 4. d Correlation between the numbers of OTR neurons and OT fibers within the same slice (n slice = 64). Each dot represents one analyzed brain section. Pearson r correlation, R 2 = 0.5745, p < 0.0001 (two-sided), slope = 0.2438. e , f Three-dimensional reconstruction of OTergic contacts with vlPAG OTR neurons. e Overview image showing OTR-neurons (green), OT-fibers (magenta), synaptophysin (SYN, red), and DAPI (blue). f Magnified images showing contacts with or without SYN. White arrowheads indicate co-localization of OT (magenta) and SYN (red), while white arrowheads with an asterisk show a mismatch of OT and SYN. DAPI = blue, OTR = green. g Bar graph showing the percentage of OTR positive ( n = 496) and negative ( n = 3840) cells receiving OT innervation (<1 µm distance between fibers and cells). n rats = 4, 8 images per animal, p = 0.0055 (two-sided). h Bar graph showings the percentage of contacts between OT and OTR-positive neurons at somatic and dendritic locations. n rats = 4, 8 images per animal, p < 0.0001 (two-sided). i Reconstruction of a vGluT2-positive (red) OT fibers (magenta) within the vlPAG. j Bar graph showing that the vast majority of OT fibers within the vlPAG are vGluT2-negative (92.4%). n = 4. k , l 3D reconstruction of contacts between an OTR dendrite and OT fibers. k Co-localization of OT (magenta) and vGluT2 (red) are indicated by white arrowheads. l Mismatch of OT (magenta) and vGluT2 (red) are indicated by white arrowheads with an asterisk. DAPI = blue, OTR = green. n = 4 female rats. Scale bars in order of appearance: 50, 10, 10, 20, and 20 µm. Results are expressed as the mean ± SEM and the individual points of each conditions are represented as white circle. Source data are provided as a Source data file.

Journal: Nature Communications

Article Title: An analgesic pathway from parvocellular oxytocin neurons to the periaqueductal gray in rats

doi: 10.1038/s41467-023-36641-7

Figure Lengend Snippet: a Schema of rAAV 1/2 -pEF1α-DIO-GFP injection in the PAG of OTR-Cre rats. b Graph showing the relation between the number of OT fibers in the vlPAG and the bregma level. Blue area represents SEM. n fibers = 290, n rats = 4. c Graph showing the relation between the percentage of OTR neurons in the vlPAG and the bregma level. Blue area represents SEM. n cells = 528, n rats = 4. d Correlation between the numbers of OTR neurons and OT fibers within the same slice (n slice = 64). Each dot represents one analyzed brain section. Pearson r correlation, R 2 = 0.5745, p < 0.0001 (two-sided), slope = 0.2438. e , f Three-dimensional reconstruction of OTergic contacts with vlPAG OTR neurons. e Overview image showing OTR-neurons (green), OT-fibers (magenta), synaptophysin (SYN, red), and DAPI (blue). f Magnified images showing contacts with or without SYN. White arrowheads indicate co-localization of OT (magenta) and SYN (red), while white arrowheads with an asterisk show a mismatch of OT and SYN. DAPI = blue, OTR = green. g Bar graph showing the percentage of OTR positive ( n = 496) and negative ( n = 3840) cells receiving OT innervation (<1 µm distance between fibers and cells). n rats = 4, 8 images per animal, p = 0.0055 (two-sided). h Bar graph showings the percentage of contacts between OT and OTR-positive neurons at somatic and dendritic locations. n rats = 4, 8 images per animal, p < 0.0001 (two-sided). i Reconstruction of a vGluT2-positive (red) OT fibers (magenta) within the vlPAG. j Bar graph showing that the vast majority of OT fibers within the vlPAG are vGluT2-negative (92.4%). n = 4. k , l 3D reconstruction of contacts between an OTR dendrite and OT fibers. k Co-localization of OT (magenta) and vGluT2 (red) are indicated by white arrowheads. l Mismatch of OT (magenta) and vGluT2 (red) are indicated by white arrowheads with an asterisk. DAPI = blue, OTR = green. n = 4 female rats. Scale bars in order of appearance: 50, 10, 10, 20, and 20 µm. Results are expressed as the mean ± SEM and the individual points of each conditions are represented as white circle. Source data are provided as a Source data file.

Article Snippet: Rabbit polyclonal anti-vGluT2 primary antibody , SYSY , 135403.

Techniques: Injection

Journal: Nature Communications

Article Title: An analgesic pathway from parvocellular oxytocin neurons to the periaqueductal gray in rats

doi: 10.1038/s41467-023-36641-7

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti-vGluT2 primary antibody , SYSY , 135403.

Techniques: Plasmid Preparation, Recombinant, Positive Control, Negative Control, Software

Kv4.2 protein domains are found in preferential contact with VGluT2 and not with VGluT1. (A–D) Triple immunofluorescent labeling for vesicular glutamate transporter 2 (VGluT2, green: A ), vesicular glutamate transporter 1 (VGluT1, cyan: B ), voltage-dependent potassium channel 4.2 (Kv4.2, magenta: C ), and merged (D) as shown in images in the adult mouse MGV. Scale bar: 5 μm. (E) The ratio of (VGluT1 or VGluT2 positive) axon terminals that are contact with Kv4.2-positive puncta in all (VGluT1 or VGluT2 positive, respectively) axon terminals is significantly higher in the VGluT2-positive excitatory axon terminals than in the VGluT1-positive terminals ( P < 0.00001, t -test). The horizontal lines in the box and whisker plots represent the median values, and the bottom and top of the boxes represent the lower and upper quartiles, respectively. The “x” represents the mean, and the bars represent the minimum and maximum values within 1.5 times the lower and upper quartiles.

Journal: Frontiers in Neuroscience

Article Title: Kv4.2-Positive Domains on Dendrites in the Mouse Medial Geniculate Body Receive Ascending Excitatory and Inhibitory Inputs Preferentially From the Inferior Colliculus

doi: 10.3389/fnins.2021.740378

Figure Lengend Snippet: Kv4.2 protein domains are found in preferential contact with VGluT2 and not with VGluT1. (A–D) Triple immunofluorescent labeling for vesicular glutamate transporter 2 (VGluT2, green: A ), vesicular glutamate transporter 1 (VGluT1, cyan: B ), voltage-dependent potassium channel 4.2 (Kv4.2, magenta: C ), and merged (D) as shown in images in the adult mouse MGV. Scale bar: 5 μm. (E) The ratio of (VGluT1 or VGluT2 positive) axon terminals that are contact with Kv4.2-positive puncta in all (VGluT1 or VGluT2 positive, respectively) axon terminals is significantly higher in the VGluT2-positive excitatory axon terminals than in the VGluT1-positive terminals ( P < 0.00001, t -test). The horizontal lines in the box and whisker plots represent the median values, and the bottom and top of the boxes represent the lower and upper quartiles, respectively. The “x” represents the mean, and the bars represent the minimum and maximum values within 1.5 times the lower and upper quartiles.

Article Snippet: Then, the sections were incubated for 3 days at 20°C with the following primary antibodies: mouse monoclonal anti-Kv4.2 antibody (1:2,500; NeuroMab, UC Davis, United States), guinea pig polyclonal anti-vesicular glutamate transporter 2 (VGluT2) antibody (1:5,000; Frontier Institute, Ishikari, Hokkaido, Japan), guinea pig polyclonal anti-VGluT1 antibody (1:5,000; Frontier), rabbit polyclonal anti-VGluT2 antibody (1:5,000; Abcam, Cambridge, United Kingdom), rabbit polyclonal anti-glutamic acid decarboxylase (GAD) 65/67 antibody (1:5,000; Sigma-Aldrich, St Louis, MO, United States), rabbit polyclonal anti-calbindin antibody (1:2,500; Sigma-Aldrich), goat polyclonal anti-calretinin antibody (1:2,500; Abcam), chicken polyclonal anti-GFP antibody (1:2,500; Abcam), and mouse monoclonal anti-neuronal nuclei (NeuN) antibody (1:5,000; Millipore, Billerica, MA, United States).

Techniques: Labeling, Whisker Assay

Kv4.2-positive processes from MGB neurons receive excitatory (VGluT2) inputs from the IC. (A) An example of the coronal section of IC showing the center of injection of Phaseolus vulgaris -leucoagglutinin (PHAL). (B–I) Triple immunofluorescence for PHAL (cyan; B,E ), VGluT2 (green; C,G ), and Kv4.2 (magenta; D,H ), in the mice MGV. (E,I) Images are acquired from different sections. Scale bars: 500 μm (A) , 5 μm (B–I) . IC, inferior colliculus; VGluT2, vesicular glutamate transporter 2.

Journal: Frontiers in Neuroscience

Article Title: Kv4.2-Positive Domains on Dendrites in the Mouse Medial Geniculate Body Receive Ascending Excitatory and Inhibitory Inputs Preferentially From the Inferior Colliculus

doi: 10.3389/fnins.2021.740378

Figure Lengend Snippet: Kv4.2-positive processes from MGB neurons receive excitatory (VGluT2) inputs from the IC. (A) An example of the coronal section of IC showing the center of injection of Phaseolus vulgaris -leucoagglutinin (PHAL). (B–I) Triple immunofluorescence for PHAL (cyan; B,E ), VGluT2 (green; C,G ), and Kv4.2 (magenta; D,H ), in the mice MGV. (E,I) Images are acquired from different sections. Scale bars: 500 μm (A) , 5 μm (B–I) . IC, inferior colliculus; VGluT2, vesicular glutamate transporter 2.

Techniques: Injection, Immunofluorescence

Kv4.2-positive processes from MGV neurons receive excitatory (VGluT2) inputs from the IC (GFP). (A) An example of a GFP-immunostained sagittal section containing IC and showing the center of injection. (B) GFP-positive axonal plexuses in the MGB. (C–R) Triple immunofluorescence for GFP (cyan; C,G,K,O ), VGluT2 (green; D,H,L,P ), and Kv4.2 (magenta; E,I,M,Q ) in the MGVof PV-Cre mice. (F,J,N,R) Images are acquired from different sections. Scale bar: 1 mm (A) , 50 μm (B) , and 2 μm (C–R) . GFP, green fluorescent protein; IC, inferior colliculus; Kv4, Shal-related subfamily of potassium voltage-gated channel; MGV, ventral medial geniculate; VGluT2, vesicular glutamate transporter 2.

Journal: Frontiers in Neuroscience

Article Title: Kv4.2-Positive Domains on Dendrites in the Mouse Medial Geniculate Body Receive Ascending Excitatory and Inhibitory Inputs Preferentially From the Inferior Colliculus

doi: 10.3389/fnins.2021.740378

Figure Lengend Snippet: Kv4.2-positive processes from MGV neurons receive excitatory (VGluT2) inputs from the IC (GFP). (A) An example of a GFP-immunostained sagittal section containing IC and showing the center of injection. (B) GFP-positive axonal plexuses in the MGB. (C–R) Triple immunofluorescence for GFP (cyan; C,G,K,O ), VGluT2 (green; D,H,L,P ), and Kv4.2 (magenta; E,I,M,Q ) in the MGVof PV-Cre mice. (F,J,N,R) Images are acquired from different sections. Scale bar: 1 mm (A) , 50 μm (B) , and 2 μm (C–R) . GFP, green fluorescent protein; IC, inferior colliculus; Kv4, Shal-related subfamily of potassium voltage-gated channel; MGV, ventral medial geniculate; VGluT2, vesicular glutamate transporter 2.

Techniques: Injection, Immunofluorescence

Formation of synapses between Kv4.2-positive and negative postsynaptic MGB microregions and VGluT2 excitatory terminals. (A,B) Examples of asymmetric excitatory synapses in the MGV using pre-embedding immunoelectron microscopy. Arrow: Kv4.2 (gold colloid), arrowhead: asymmetrical synapse that is in the contact with Kv4.2-positive postsynaptic structure, double arrowhead: asymmetrical synapse that is in the contact with Kv4.2-negative postsynaptic structure, and double arrow: symmetrical synapse that is in the contact with Kv4.2-positive postsynaptic structure. (C) The area of axon terminals of those asymmetric synapses that are in contact with gold particles positive or negative postsynaptic structure. Terminals forming asymmetric synapse with Kv4.2-positive MGV dendrites are significantly larger than those forming synapses with Kv4.2-negative MGV dendrites ( P < 0.00001, t -test). Scale bar: 1 μm (A,B) . The horizontal lines in the box and whisker plots represent the median values, and the bottom and top of the boxes represent the lower and upper quartiles, respectively. The “x” represents the mean and the bars represent the minimum and maximum values within 1.5 times the lower and upper quartiles. Kv4, Shal-related subfamily of potassium voltage-gated channel; MGV, ventral medial geniculate.

Journal: Frontiers in Neuroscience

Article Title: Kv4.2-Positive Domains on Dendrites in the Mouse Medial Geniculate Body Receive Ascending Excitatory and Inhibitory Inputs Preferentially From the Inferior Colliculus

doi: 10.3389/fnins.2021.740378

Figure Lengend Snippet: Formation of synapses between Kv4.2-positive and negative postsynaptic MGB microregions and VGluT2 excitatory terminals. (A,B) Examples of asymmetric excitatory synapses in the MGV using pre-embedding immunoelectron microscopy. Arrow: Kv4.2 (gold colloid), arrowhead: asymmetrical synapse that is in the contact with Kv4.2-positive postsynaptic structure, double arrowhead: asymmetrical synapse that is in the contact with Kv4.2-negative postsynaptic structure, and double arrow: symmetrical synapse that is in the contact with Kv4.2-positive postsynaptic structure. (C) The area of axon terminals of those asymmetric synapses that are in contact with gold particles positive or negative postsynaptic structure. Terminals forming asymmetric synapse with Kv4.2-positive MGV dendrites are significantly larger than those forming synapses with Kv4.2-negative MGV dendrites ( P < 0.00001, t -test). Scale bar: 1 μm (A,B) . The horizontal lines in the box and whisker plots represent the median values, and the bottom and top of the boxes represent the lower and upper quartiles, respectively. The “x” represents the mean and the bars represent the minimum and maximum values within 1.5 times the lower and upper quartiles. Kv4, Shal-related subfamily of potassium voltage-gated channel; MGV, ventral medial geniculate.

Techniques: Immuno-Electron Microscopy, Whisker Assay

Some of Kv4.2-positive domains receive contact with both VGluT2 excitatory and GABAergic inhibitory axon terminals. (A–H) Triple fluorescence labeling for VGluT2 (green; A,E ), GAD65/67 (cyan; B,F ), and Kv4.2 (magenta; C,G ) in the mice MGV. Arrowhead: Kv4.2-positive structure that is closely opposite to both GAD65/67-positive terminal and VGluT2-positive terminal. Double arrowhead: Kv4.2-positive structures that are closely opposite to VGluT2-positive terminals but not to GAD65/67-positive terminals. (I) A summary schematic diagram representing the relationship between Kv4.2-positive structures and glutamatergic and GABAergic inputs from the IC, RTN, and cortex. Scale bar: 1 μm (A–H) . GABA, gamma-aminobutyric acid; GAD, glutamic acid decarboxylase; IC, inferior colliculus; Kv4, Shal-related subfamily of potassium voltage-gated channel; MGV, ventral medial geniculate; RTN, reticular thalamic nucleus; VGluT2, vesicular glutamate transporter 2.

Journal: Frontiers in Neuroscience

Article Title: Kv4.2-Positive Domains on Dendrites in the Mouse Medial Geniculate Body Receive Ascending Excitatory and Inhibitory Inputs Preferentially From the Inferior Colliculus

doi: 10.3389/fnins.2021.740378

Figure Lengend Snippet: Some of Kv4.2-positive domains receive contact with both VGluT2 excitatory and GABAergic inhibitory axon terminals. (A–H) Triple fluorescence labeling for VGluT2 (green; A,E ), GAD65/67 (cyan; B,F ), and Kv4.2 (magenta; C,G ) in the mice MGV. Arrowhead: Kv4.2-positive structure that is closely opposite to both GAD65/67-positive terminal and VGluT2-positive terminal. Double arrowhead: Kv4.2-positive structures that are closely opposite to VGluT2-positive terminals but not to GAD65/67-positive terminals. (I) A summary schematic diagram representing the relationship between Kv4.2-positive structures and glutamatergic and GABAergic inputs from the IC, RTN, and cortex. Scale bar: 1 μm (A–H) . GABA, gamma-aminobutyric acid; GAD, glutamic acid decarboxylase; IC, inferior colliculus; Kv4, Shal-related subfamily of potassium voltage-gated channel; MGV, ventral medial geniculate; RTN, reticular thalamic nucleus; VGluT2, vesicular glutamate transporter 2.

Techniques: Fluorescence, Labeling

Focal injection using iontophoresis (A) A retrograde tracer, Alexa Fluor 647-conjugated cholera toxin subunit B (CTB647, 0.2% in ddH 2 O), is iontophoretically delivered to the dorsal part of the thalamic ventral posteromedial nucleus (VPM). VPM is identified by VGLUT2 immunohistochemistry using a guinea pig anti-VGluT2 antibody and an Alexa Fluor 488-conjugated secondary antibody. Gray-scale images are acquired using an epifluorescence microscope (Axio Scope.A1, Carl Zeiss) equipped with a cooled CCD camera (QSI RS 6.1, Quantum Scientific Imaging) and pseudocolored using Adobe Photoshop software. D, dorsal; L, lateral; VPL, ventral posterolateral nucleus. Scale bar, 0.5 mm. (B) Retrogradely labeled cells in the ventral part of the principal trigeminal nucleus of the brainstem (Pr5) in the contralateral hemisphere. M, medial. Scale bar, 0.5 mm.

Journal: STAR Protocols

Article Title: Electrophysiological and anatomical characterization of synaptic remodeling in the mouse whisker thalamus

doi: 10.1016/j.xpro.2021.100743

Figure Lengend Snippet: Focal injection using iontophoresis (A) A retrograde tracer, Alexa Fluor 647-conjugated cholera toxin subunit B (CTB647, 0.2% in ddH 2 O), is iontophoretically delivered to the dorsal part of the thalamic ventral posteromedial nucleus (VPM). VPM is identified by VGLUT2 immunohistochemistry using a guinea pig anti-VGluT2 antibody and an Alexa Fluor 488-conjugated secondary antibody. Gray-scale images are acquired using an epifluorescence microscope (Axio Scope.A1, Carl Zeiss) equipped with a cooled CCD camera (QSI RS 6.1, Quantum Scientific Imaging) and pseudocolored using Adobe Photoshop software. D, dorsal; L, lateral; VPL, ventral posterolateral nucleus. Scale bar, 0.5 mm. (B) Retrogradely labeled cells in the ventral part of the principal trigeminal nucleus of the brainstem (Pr5) in the contralateral hemisphere. M, medial. Scale bar, 0.5 mm.

Article Snippet: Rabbit Polyclonal Anti-VGluT2 Antibody (1:500–1,000 dilution) , Nittobo Medical Co., Ltd. (transferred from Frontier Institute Co., Ltd.) , Cat#MSFR106310; PRID: AB_2619683.

Techniques: Injection, Immunohistochemistry, Microscopy, Imaging, Software, Labeling

Post hoc visualization of cell morphology after electrophysiological recording A VPM neuron is visualized with Alexa Fluor 488-conjugated streptavidin. Immunohistochemistry against vesicular glutamate transporter type 2 (VGluT2) and Alexa Fluor 647-conjugated secondary antibody is simultaneously performed. Gray-scale images are acquired using a fluorescent microscope (BZ-X810, Keyence) and pseudocolored. The slice is resectioned at 40 μm. Scale bar, 20 μm.

Journal: STAR Protocols

Article Title: Electrophysiological and anatomical characterization of synaptic remodeling in the mouse whisker thalamus

doi: 10.1016/j.xpro.2021.100743

Figure Lengend Snippet: Post hoc visualization of cell morphology after electrophysiological recording A VPM neuron is visualized with Alexa Fluor 488-conjugated streptavidin. Immunohistochemistry against vesicular glutamate transporter type 2 (VGluT2) and Alexa Fluor 647-conjugated secondary antibody is simultaneously performed. Gray-scale images are acquired using a fluorescent microscope (BZ-X810, Keyence) and pseudocolored. The slice is resectioned at 40 μm. Scale bar, 20 μm.

Article Snippet: Rabbit Polyclonal Anti-VGluT2 Antibody (1:500–1,000 dilution) , Nittobo Medical Co., Ltd. (transferred from Frontier Institute Co., Ltd.) , Cat#MSFR106310; PRID: AB_2619683.

Techniques: Immunohistochemistry, Microscopy

Journal: STAR Protocols

Article Title: Electrophysiological and anatomical characterization of synaptic remodeling in the mouse whisker thalamus

doi: 10.1016/j.xpro.2021.100743

Figure Lengend Snippet:

Article Snippet: Rabbit Polyclonal Anti-VGluT2 Antibody (1:500–1,000 dilution) , Nittobo Medical Co., Ltd. (transferred from Frontier Institute Co., Ltd.) , Cat#MSFR106310; PRID: AB_2619683.

Techniques: Recombinant, Liposomes, Refractive Index, Plasmid Preparation, Staining, Ointment, Software, Laser-Scanning Microscopy, Imaging, Microscopy, Fluorescence, Injection

Journal: eLife

Article Title: Localization, proteomics, and metabolite profiling reveal a putative vesicular transporter for UDP-glucose

doi: 10.7554/eLife.65417

Figure Lengend Snippet:

Article Snippet: Antibody , Polyclonal rabbit anti-VGLUT2 , Synaptic Systems , Cat. #: 135402 RRID: AB_2187539 , WB dilution 1:1000.

Techniques: Biomarker Discovery, Recombinant, Plasmid Preparation

Journal: Brain structure & function

Article Title: Thalamic Degeneration in MPTP-treated Parkinsonian Monkeys: Impact upon Glutamatergic Innervation of Striatal Cholinergic Interneurons

doi: 10.1007/s00429-019-01967-w

Figure Lengend Snippet: Control and MPTP-treated monkeys used

Article Snippet: - vGluT2 antibody: A rabbit anti-human vGluT2 polyclonal antibody (Mab Technologies) was used.

Techniques: Control

Journal: Brain structure & function

Article Title: Thalamic Degeneration in MPTP-treated Parkinsonian Monkeys: Impact upon Glutamatergic Innervation of Striatal Cholinergic Interneurons

doi: 10.1007/s00429-019-01967-w

Figure Lengend Snippet: Antibody information

Article Snippet: - vGluT2 antibody: A rabbit anti-human vGluT2 polyclonal antibody (Mab Technologies) was used.

Techniques: Purification

Electron micrographs of ChAT-immunostained (peroxidase) dendrites and vGluT2-immunolabeled (silver-intensified gold particles) terminals in the putamen (a, b, d, e) and caudate (c, f) of control (a, b, c) and MPTP-treated monkeys (d, e, f). (a) A large-sized ChAT+ dendrite (diameter >1μm) forms an asymmetric synapse (white arrows) with a vGluT2-negative (vGluT2-) terminal. In the same field, some vGluT2-positive (vGluT2+) terminals form asymmetric synapses (black arrows) with non-labeled dendrites (d) and a dendritic spine (sp). (b) A vGluT2+ terminal forms an asymmetric synapse with a small-sized (diameter <0.5μm) ChAT+ dendrite (double white arrows). (c) Medium-sized (diameter between 0.5–1μm) ChAT-immunostained dendrite receiving an asymmetric synapse from a vGluT2-negative (white arrows). (d-f) Large- (d) and medium-sized (e, f) ChAT+ dendrites form asymmetric synapses with vGluT2- (white arrows in d and f) and vGluT2+ (double white arrows in e) terminals. Scale bars in a = 0.5μm, in b (applies to c) = 0.5 μm and in d = 0.5μm (applies to d and f)

Journal: Brain structure & function

Article Title: Thalamic Degeneration in MPTP-treated Parkinsonian Monkeys: Impact upon Glutamatergic Innervation of Striatal Cholinergic Interneurons

doi: 10.1007/s00429-019-01967-w

Figure Lengend Snippet: Electron micrographs of ChAT-immunostained (peroxidase) dendrites and vGluT2-immunolabeled (silver-intensified gold particles) terminals in the putamen (a, b, d, e) and caudate (c, f) of control (a, b, c) and MPTP-treated monkeys (d, e, f). (a) A large-sized ChAT+ dendrite (diameter >1μm) forms an asymmetric synapse (white arrows) with a vGluT2-negative (vGluT2-) terminal. In the same field, some vGluT2-positive (vGluT2+) terminals form asymmetric synapses (black arrows) with non-labeled dendrites (d) and a dendritic spine (sp). (b) A vGluT2+ terminal forms an asymmetric synapse with a small-sized (diameter <0.5μm) ChAT+ dendrite (double white arrows). (c) Medium-sized (diameter between 0.5–1μm) ChAT-immunostained dendrite receiving an asymmetric synapse from a vGluT2-negative (white arrows). (d-f) Large- (d) and medium-sized (e, f) ChAT+ dendrites form asymmetric synapses with vGluT2- (white arrows in d and f) and vGluT2+ (double white arrows in e) terminals. Scale bars in a = 0.5μm, in b (applies to c) = 0.5 μm and in d = 0.5μm (applies to d and f)

Article Snippet: - vGluT2 antibody: A rabbit anti-human vGluT2 polyclonal antibody (Mab Technologies) was used.

Techniques: Immunolabeling, Control, Labeling

Electron micrographs of vGluT2-positive (vGluT2+) terminals forming asymmetric synapses with dendrites (d) and dendritic spines (sp) in the caudate (b, c) and putamen (a, d) of control (a, b) and MPTP-treated-parkinsonian monkeys (c, d). In the same field, vGluT2- negative (vGluT2-) terminals form asymmetric synapses with dendritic spines. Scale bar in a (applies to b and c) and in d = 0.5μm

Journal: Brain structure & function

Article Title: Thalamic Degeneration in MPTP-treated Parkinsonian Monkeys: Impact upon Glutamatergic Innervation of Striatal Cholinergic Interneurons

doi: 10.1007/s00429-019-01967-w

Figure Lengend Snippet: Electron micrographs of vGluT2-positive (vGluT2+) terminals forming asymmetric synapses with dendrites (d) and dendritic spines (sp) in the caudate (b, c) and putamen (a, d) of control (a, b) and MPTP-treated-parkinsonian monkeys (c, d). In the same field, vGluT2- negative (vGluT2-) terminals form asymmetric synapses with dendritic spines. Scale bar in a (applies to b and c) and in d = 0.5μm

Article Snippet: - vGluT2 antibody: A rabbit anti-human vGluT2 polyclonal antibody (Mab Technologies) was used.

Techniques: Control

Histograms comparing the density of vGluT2-positive terminals in the caudate (a) and putamen (b) of control (N=3) and MPTP-treated (N=3) parkinsonian monkeys. This analysis revealed that the density of vGluT2+ terminals forming synapses onto dendrites and spines in both the head and body of the caudate, and the pre- and post-commissural putamen was not statistically different between control and MPTP-treated monkeys (t-test, p values: Caudate head axo-spine=0.442; axo-dendrite= 0.145. Caudate body axo-spine=0.0961; axo-dendrite=0.0474. Pre-commissural putamen axo-spine=0.066; axo-dendrite=0.035. Postcommissural putamen axo-spine=0.516; axo-dendrite=0.486

Journal: Brain structure & function

Article Title: Thalamic Degeneration in MPTP-treated Parkinsonian Monkeys: Impact upon Glutamatergic Innervation of Striatal Cholinergic Interneurons

doi: 10.1007/s00429-019-01967-w

Figure Lengend Snippet: Histograms comparing the density of vGluT2-positive terminals in the caudate (a) and putamen (b) of control (N=3) and MPTP-treated (N=3) parkinsonian monkeys. This analysis revealed that the density of vGluT2+ terminals forming synapses onto dendrites and spines in both the head and body of the caudate, and the pre- and post-commissural putamen was not statistically different between control and MPTP-treated monkeys (t-test, p values: Caudate head axo-spine=0.442; axo-dendrite= 0.145. Caudate body axo-spine=0.0961; axo-dendrite=0.0474. Pre-commissural putamen axo-spine=0.066; axo-dendrite=0.035. Postcommissural putamen axo-spine=0.516; axo-dendrite=0.486

Article Snippet: - vGluT2 antibody: A rabbit anti-human vGluT2 polyclonal antibody (Mab Technologies) was used.

Techniques: Control

Histograms comparing the percentages of ChAT-positive dendrites that form asymmetric synapses with vGluT2-positive terminals in the caudate and putamen of control and MPTP-treated monkeys. In both the caudate and putamen, no significant differences (Chi-square test) between control and parkinsonian monkeys were found in the proportions of ChAT-positive dendrites receiving vGluT2-positive terminals

Journal: Brain structure & function

Article Title: Thalamic Degeneration in MPTP-treated Parkinsonian Monkeys: Impact upon Glutamatergic Innervation of Striatal Cholinergic Interneurons

doi: 10.1007/s00429-019-01967-w

Figure Lengend Snippet: Histograms comparing the percentages of ChAT-positive dendrites that form asymmetric synapses with vGluT2-positive terminals in the caudate and putamen of control and MPTP-treated monkeys. In both the caudate and putamen, no significant differences (Chi-square test) between control and parkinsonian monkeys were found in the proportions of ChAT-positive dendrites receiving vGluT2-positive terminals

Article Snippet: - vGluT2 antibody: A rabbit anti-human vGluT2 polyclonal antibody (Mab Technologies) was used.

Techniques: Control

Review

Structured Review

Images

1) Product Images from "Loss of Bmal1 impairs the glutamatergic light input to the SCN in mice"

Similar Products

Image Search Results